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. 2018 Apr 12;102(5):985–994. doi: 10.1016/j.ajhg.2018.03.004

Figure 3.

Figure 3

Expression Analysis of NAA15 in Research-Subject-Derived Cell Lines

(A and D) Sanger sequencing of genomic DNA (top panel) and reverse-transcribed cDNA (bottom panel) isolated from a lymphoblastoid cell line (LCL) of individual 10-1 (c.239_240delAT) (A) and an induced pluripotent stem cell (iPS) line (passage 16) of individual 19 (c.1009_1012delGAAA) (D).

(B and E) Quantification of different cDNA species from cDNA Sanger sequencing showing the relative ratio of WT NAA15 versus c.239_240delAT (LCL line) (B) and (c.1009_1012delGAAA) (passage 16 iPS cell line) (E).

(C and F) NAA15 mRNA expression level analyzed by qPCR in research subject-derived cell lines (at passage numbers p10, p13, and p16), as compared to control cell lines (at passage 16). Error bars are standard deviation (SD), and the assay was performed three times per sample.