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Truncation Mutations of Human NAA15 Impair NatA Function and Yeast Viability
(A) Serial dilution spot assay depicting the sensitivity of human NAA15 Thr55Hisfs∗2 and Lys305∗ mutants to increased temperature in a ynaa10Δ, ynaa15Δ double-deletion background (yNatAΔ).
(B) Confirmation of human NatA expression by immunoblot analysis with anti-hNAA10 and anti-HA (for HA-hNAA15 detection) along with anti-beta Actin as a loading control.
