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. 2018 May 25;14:168–177. doi: 10.1016/j.bbrep.2018.04.010

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — UC and SEP aggregates fractions for LC-MS/MS analysis. (A) SDS-PAGE showing gel bands dissected for in-gel Trypsin digestion for LC-MS/MS (a total of 15 for both UC and SEP) (same picture shown in A); (B) Protein Identification performed with Proteome Discoverer 1.4 generated 369 and 605 proteins unique to UC and SEP respectively; 225 were shared by the two extraction methods (2 peptides for protein as minimum and a peptide count only in the top scored proteins were utilized). UC: ultracentrifugation; SEP: Seprion extraction pads.