Table 1.
Conditions for aggregate enrichment by Seprion Magnetic PAD-Beads: volumes of samples and reagents; CB: Capture Buffer; SR: Seprion-PAD Reagent.
Seprion Enrichment Condition |
|||||||
---|---|---|---|---|---|---|---|
Reagent | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
Sample [µl] | 800 | 400 | 400 | 400 | 400 | 400 | 200 |
CB [µl] | 200 | 400 | 200 | 200 | 400 | 400 | 200 |
Water [µl] | 0 | 0 | 200 | 200 | 0 | 0 | 400 |
SR [µl] | 100 | 200 | 100 | 100 | 100 | 100 | 100 |
Beads [µl] | 100 | 200 | 200 | 100 | 200 | 100 | 100 |