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. 2018 Feb 27;26(6):1552–1567. doi: 10.1016/j.ymthe.2018.02.022

Figure 1.

Figure 1

Overexpressed TFEB Translocates to the Nucleus and Increases Autophagy-Lysosomal Markers

(A) Tfeb cDNA levels measured by qPCR in ventral midbrain homogenates of mice after vehicle (n = 6) or AAV-TFEB nigral injection (n = 5); Mann-Whitney test. *p < 0.05 compared to control. (B) Immunofluorescence for tyrosine hydroxylase (red), Flag (green), and nucleus (blue) in substantia nigra sections showing 3xFlag-TFEB translocation to the nucleus after AAV-TFEB nigral injection. Scale bar, 25 μm. (C–E) Representative western blots and protein levels in ventral midbrain homogenates from mice overexpressing TFEB (n = 6) compared to vehicle-injected mice (n = 5–6) of (C) LAMP1, (D) CTSD, and (E) LC3-I and -II. Mann-Whitney test, *p < 0.05 compared to control. In all panels, samples were collected 5 weeks after AAV-TFEB or vehicle injections. All data are represented as mean ± SEM.