Figure 3.

Activation of EIF4E/4E-BP1 and S6K1 Protein Synthesis Pathways

(A–C) Representative western blots and protein levels in ventral midbrain homogenates from mice overexpressing TFEB (n = 6) compared to control mice (n = 5 to 6) of (A) EIF4E, (B) phosphorylated and non-phosphorylated 4E-BP1, p4E-BP1^S65, expressed as a ratio, and (C) endogenous ribosomal protein S6K1, its activated form (pS6K1^T389), and the ratio. Mann-Whitney test, *p < 0.05 compared to control. (D) Representative photomicrograph of a substantia nigra section immunostained for the phosphorylated/activated RPS6, pRPS6^S235/236, of a mouse injected with AAV-TFEB into the right substantia nigra. Scale bar, 50 μm. In all panels, samples were collected 5 weeks after AAV-TFEB or vehicle injections. All data are represented as mean ± SEM.