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. 2018 Mar 19;26(6):1568–1580. doi: 10.1016/j.ymthe.2018.03.012

Figure 1.

Figure 1

MAC-Mediated NLRP3 Inflammasome Activation Increases IL-1β Production in EAU

(A) Representative retinal cryostat sections stained with C5b-9 antibody, indicating elevated MAC formation in EAU retina relative to normal retina. As anticipated, C9−/− EAU mouse retina remained negative for MAC. For quantification of MAC fluorescence intensity, refer to Figure S1. (B and C) Production of IL-1β, as measured by ELISA (B) and real-time PCR (C) in EAU retinas, was significantly higher than normal control retinas. However, moderate increases in IL-1β protein and mRNA levels in C9−/− EAU retinas were found relative to normal control retinas. (D and E) Western blot indicated an increase in NLRP3 (D) and Caspase-1 (p20) (E) protein expression in EAU retinas relative to normal control retinas. However, a small increase was recorded in C9−/− EAU retinas relative to normal control retinas. (F) Western blot indicating an increase in ASC protein expression in EAU retinas relative to normal control retinas. However, the levels of ASC in C9−/− EAU mice retinas were only marginally higher relative to normal control retinas. The western blot and real-time PCR values were normalized to β-actin. Values are represented as mean ± SEM. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segments; scale bar, 100 μm.