Figure 8.

Effect of sCD59 on OCT Changes and Retinal Histology Score in EAU

(A) Horizontal OCT scans showing retinal foldings in the retina (red arrow) and vitreous cellular infiltrates (white arrow) in AAVCAGGFP-injected or AAVCAGsCD59-injected or PBS-injected EAU mice. (B) Retinal sections (5 μm) were generated from paraffin-embedded eyes on day 24 post-EAU induction and stained with H&E. Infiltration of inflammatory immune cells in the vitreous (V) are shown by black arrows; white asterisk represents retinal (R) detachment; retinal folds are shown by white arrowheads. (C–E) Severity of EAU pathology is represented by individual infiltration (C), vasculitis (D), and photoreceptor damage (E) as described in the Materials and Methods. Values are represented as mean ± SEM. RPE-CH, RPE and choroid; R, retina; V, vitreous; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segments; OpN, optic nerve. Scale bar, 100 μm.