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. 2018 Mar 28;26(6):1447–1456. doi: 10.1016/j.ymthe.2018.03.016

Figure 5.

Figure 5

Specificity of BCMA(171)

Vectors containing either (1) BCMA-targeting scFvs with C-terminal mCherry or (2) TNFR and immunoglobulin (Ig) superfamily members with C-terminal GFP were transiently transfected into different populations of HEK293s cells. Potential scFv/target antigen interaction was identified as a double-positive signal by automated flow cytometry. Peaks labeled “B” indicate interactions with BCMA+ controls. Unlike scFv clone 130, which interacted with two isoforms of SIRP-B1 (Figure S2A), all other scFvs, including clone 171 (shown), interacted only with BCMA.