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. 2018 Jun 4;9(6):675. doi: 10.1038/s41419-018-0591-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Western blot assay of TCF4 and LEF1 expression in HCC cells overexpressing DDX39 transfected with siRNAs for TCF4 or LEF1. b qRT-PCR analyzed the expression of Wnt/β-catenin target genes like MYC, CD44, RUNX2, SNAIL1, and CCND1 in HCC cells overexpressing DDX39 transfected with siRNAs for TCF4 or LEF1. c Transwell assay of the effect of TCF4 or LEF1 knockdown in HCC cells overexpressing DDX39 on cell invasion. d 3D spheroid invasion assay of the effect of TCF4 or LEF1 knockdown in HCC cells overexpressing DDX39 on cell invasion. Data are shown as means ± SD. *p < 0.05