Fig. 3. Untargeted metabolomics and multivariate statistical analysis of tumor tissues from CRC male mice using UHPLC-QTOF in positive ion mode disclosed significant metabolic alternations caused by SNX10 deficiency.

a Total ion chromatograms of WT (WTM), SNX10 KO (KOM), and quality control samples (QC). b Retention time deviation of all samples. c Manhattan map showed the distribution of significant features (P < 0.05, green dots) in m/z and retention time. d 2D score plot between the selected PCs of PLS-DA model. e 3D score plot between the selected PCs of PCA model. f Volcano plot with fold change threshold and t-test threshold to select the important features