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. 2018 Jun 4;9(6):676. doi: 10.1038/s41419-018-0703-x

Fig. 2. Mitochondrial COMP maintains the contractile phenotype of VSMCs.

Fig. 2

a Systematic view of mitochondrial transplantation. Mito, mitochondria. b Mitochondrial DNA copy number of VSMCs treated with ethidium bromide for two weeks. The data was analyzed using one-way ANOVA followed by the Student–Newman–Keuls test for post-hoc comparison and presented as the means ± SD of five independent experiments. *P < 0.05. c Live-cell confocal imaging of VSMCs after incubation with GFP-labeled mitochondria prepared from VSMCs (upper), purified GFP (middle) or vehicle (lower). Scale bar = 20 μm. d Scrambled siRNA-transfected and COMP siRNA-transfected VSMCs transplanted with scrambled or COMP siRNA-transfected mitochondria, underwent western blot analysis and quantification of the protein levels of α-actin, calponin and SM22. The data were analyzed using one-way ANOVA followed by the Student–Newman–Keuls test for post-hoc comparison and presented as the means ± SD of six independent experiments. Cyto+Mito+ indicates control VSMCs transplanted with control mitochondria, Cyto+Mito- indicates control VSMCs transplanted with COMP-deficient mitochondria, Cyto-Mito+ indicates COMP-deficient VSMCs transplanted with control mitochondria, Cyto-Mito- indicates COMP-deficient VSMCs transplanted with COMP-deficient mitochondria. *P < 0.05