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. 2018 Jun 4;8:8533. doi: 10.1038/s41598-018-26954-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Application of the different reporting thresholds developed for detection of suspicions of avian influenza outbreaks in layers. The figure shows an example of these thresholds applied for the detection of a low pathogenic outbreak in a commercial layer flock with an indoor (barn) production system. From up to bottom, the first graph shows both the CUSUM-mortality (blue dotted lines) thresholds and the fixed threshold of 0.08% mortality (green dotted line). The second graph shows the ratio-mortality threshold >2.9 (orange dotted line), the third graph shows the CUSUM-egg production thresholds and the fourth (bottom) graph shows the ratio-egg production <0.94 threshold. Points coloured in red or green (first graph marking detection using the fixed mortality threshold) are detected alarms. True alarms are those detected later than day 200 (vertical dotted line) of production.