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. 2018 Jun 4;9(6):674. doi: 10.1038/s41419-018-0718-3

Fig. 1. AGEs-induced apoptosis in osteoblastic MC3T3-E1 cells.

Fig. 1

a Cell viability was determined by MTT reduction in osteoblastic cells in the presence of AGEs. Error bars indicate SEM (n = 6). b, c Flow cytometric quantification of apoptosis. Error bars indicate SEM (n = 6). d, e TUNEL staining and assay. Error bars indicate SEM (n = 300). Scale bars = 100 μm. f Representative immunoreactive bands for Bcl-2 and Bax in osteoblastic cells in the presence of AGEs. Full-length blots are presented in Supplementary Figure 4. g Quantification of immunoreactive bands for Bcl-2 and Bax relative to β-actin. Error bars indicate SEM (n = 6)