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. 2018 Jun 4;9(6):674. doi: 10.1038/s41419-018-0718-3

Fig. 7. Silibinin protects AGEs-induced osteoblastic MC3T3-E1 cells apoptosis through modulation of RAGE.

Fig. 7

a, b Representative immunoreactive bands and relative levels of RAGE in osteoblastic cells with (+) or without (−) silibinin treatment in the presence of AGEs (+) or culture medium (−). c Cell viability determined by MTT reduction in osteoblastic cells treated with (+) or without (−) silibinin in the presence of AGEs (+). Error bars indicate SEM (n = 6). d Hoechst staining assay in the indicated groups. Error bars indicate SEM (n = 300). e, f Representative immunoreactive bands and relative levels of RAGE in osteoblastic cells transfected with plasmids carrying empty vectors or RAGE genes. g Representative immunoreactive bands for RAGE, Bcl-2, and Bax in in the indicated groups; h–j Quantification of immunoreactive bands for RAGE, Bcl-2, and Bax relative to β-actin. Error bars indicate SEM (n = 6). Full-length blots are presented in Supplementary Figure 4