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. 2018 Jun 4;8:8527. doi: 10.1038/s41598-018-26937-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of estradiol via ERα on histomorphometry and cell proliferation of mandibular condylar fibrocartilage from 7-week and 17-week old WT and ERαKO female mice after sham, ovariectomy plus placebo treatment, and ovariectomy plus estradiol treatment. Data from 7-week old mice: (A) Timeline for experiment (B) Representative hematoxylin and eosin images of all groups. (C) Cartilage thickness and (D). Cell numbers determined by histomorphometry. (E) Representative BrdU images illustrating cell proliferation and (F). Quantification of percentage of BrdU+ cells. Data from 17-week old mice. (G) Timeline for experiment. (H) Representative hematoxylin and eosin images of all groups (I) Cartilage thickness and (J). Cell numbers determined by histomorphometry. (K) Representative BrdU images illustrating cell proliferation and (L). Quantification of percentage of BrdU+ cells. All values represent means ± standard deviation. n = 6 for all data, ^{^}p < 0.01.