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. 2018 Jun 4;8:8527. doi: 10.1038/s41598-018-26937-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of estradiol via ERα on chondrogenesis in the mandibular condylar fibrocartilage of 7-week and 17-week old female mice. (A) Schematic illustrating distinct cell phenotypes and respective matrix production in the mandibular condylar fibrocartilage (B) qPCR gene expression of Sox9, Pthrp, Ihh, Col2, and Col10 from 7-week old mice (C) qPCR gene expression of Sox9, Pthrp, Ihh, Col2, and Col10 from 17-week old mice. (D) Representative Col2 immunohistochemical images and (E). Representative safranin-O images illustrating proteoglycans from 7-week old mice. (F) Representative Col2 immunohistochemical images and (G). Representative safranin-O images from 17-week old mice. All values represent means ± standard deviation. n = 6 for all data, ^{^}p < 0.01, ^*p < 0.05.