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. 2018 Jun 4;8:8529. doi: 10.1038/s41598-018-26897-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the Pachycereus pringlei transcriptome. (a) Principal component analysis of P. pringlei developmental stages and Arabidopsis root zones and (b–d) the empirical detection of gene expression shows that the transcriptional state of the P. pringlei root tip changes during development. Only 66 differentially expressed transcripts (DETs) were identified in the comparison of the Initial vs Intermediate developmental stages (b), while 873 and 1,388 DETs were identified between the Intermediate vs Terminal (b,c), and Initial vs Terminal (b,d) stages. Colors in c and d correspond to the fold change (FC): blue, FC > 4; red, 3 < FC < 4; yellow, 2 < FC < 3. In all cases, the FDR p-value was ≤0.005. IN: Initial stage; IT: Intermediate stage; TR: Terminal stage. Upregulated and downregulated refer to the expression level at the developmental stage shown in bold.