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. 2018 Jun 4;8:8514. doi: 10.1038/s41598-018-26936-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell-based activity assay of erythropoietin (EPO). (A) Growth curves of UT-7 (DSMZ-No. ACC-137) cell-line supplemented with cell-free synthesized EPO (continuous line), cell-free no-template-control (NTC, grey-dashed line), NTC + GM-CSF (dash-dot line) and commercially available hEPO (Sigma-Aldrich, #H5166-10 UG, dotted line). The concentration of cell-free produced EPO was adjusted according to the total protein concentration determined by TCA-precipitation. EPO as well as commercially purchased hEPO induced cell-growth within 4 days of cultivation. The NTC lead to a continuous decrease of living cells. (B) Analysis of UT-7 cells by light microscopy. Cells were visualized after four days of cultivation.