Figure 2.

Kinetics of ARR gene induction. A, Northern-blot analysis of 15 μg of total RNA from 3-d-old etiolated seedlings treated with 5 μm BA for various times (indicated in minutes above each lane) and hybridized with the indicated ARR probe (at left). The β-tubulin shown is a representative image and is not the loading control for all of the blots (see methods). B, Quantification of transcript levels from blot depicted in A. The signals from the northern blots were quantified with a PhosphorImager and normalized to the β-tubulin loading control. The highest level of expression for each probe was assigned a value of 100% and all other points were normalized to it (percentage of maximum).