Figure 5.

Identification of the transcription initiation site of the ARR5 gene. Primer extension analysis. A [³²P]-labeled ARR5 primer (see “Materials and Methods”) was hybridized with 50 μg of total RNA and extended using reverse transcriptase. The product was resolved on a sequencing gel (far left lane), and the mobility compared with an ARR5 genomic DNA sequencing reaction using the same ARR5 primer (lanes A, C, G, and T). The nucleotide sequence in the vicinity of the start site of transcription is shown to the right of the gel insert. The arrow marks the deduced transcription start site and the boxed residues mark the predicted TATA box. Below the gel inset is a schematic diagram of the ARR5 primer relative to the transcription and translation start sites and the primer extended product.