Table I.
Effect of cycloheximide treatment on ARR gene expression in response to cytokinin
Gene | Control | +BA | +Chxa | +BA and +Chx |
---|---|---|---|---|
ARR4 | 25 | 53 | 74 | 100 |
ARR5 | ndb | 48 | 74 | 100 |
ARR6 | 15 | 58 | 46 | 100 |
ARR7 | nd | 41 | 64 | 100 |
ARR8 | 20 | 27 | 59 | 100 |
ARR9 | 45 | 39 | 51 | 100 |
ARR15 | nd | 32 | 59 | 100 |
ARR16 | nd | nd | 51 | 100 |
Seedlings were treated with nothing (control) or a combination of 50 μm cycloheximide and subsequently BA as described in “Materials and Methods” and the total RNA was analyzed by northern blotting. The blots were hybridized to the indicated probes. The intensity of each band was quantified using a phosphor imager and the relative intensity was normalized to a tubulin loading control. The highest signal for each probing from each treatment was assigned a value of 100% and the others are presented as a percentage of this maximum.
Chx, Cycloheximide.
nd, No signal detected above the background.