Skip to main content
. 2000 Dec;124(4):1706–1717. doi: 10.1104/pp.124.4.1706

Table I.

Effect of cycloheximide treatment on ARR gene expression in response to cytokinin

Gene Control +BA +Chxa +BA and +Chx
ARR4 25 53 74 100
ARR5  ndb 48 74 100
ARR6 15 58 46 100
ARR7 nd 41 64 100
ARR8 20 27 59 100
ARR9 45 39 51 100
ARR15 nd 32 59 100
ARR16 nd nd 51 100

Seedlings were treated with nothing (control) or a combination of 50 μm cycloheximide and subsequently BA as described in “Materials and Methods” and the total RNA was analyzed by northern blotting. The blots were hybridized to the indicated probes. The intensity of each band was quantified using a phosphor imager and the relative intensity was normalized to a tubulin loading control. The highest signal for each probing from each treatment was assigned a value of 100% and the others are presented as a percentage of this maximum.

a

Chx, Cycloheximide. 

b

nd, No signal detected above the background.