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. 2018 Jun 4;9(6):667. doi: 10.1038/s41419-018-0720-9

Fig. 4. Colocalization of human immune cells with human hepatocytes in the liver of LTH hu-mice.

Fig. 4

a, b Serial sections of liver tissues from LTH hu-mice at approximately 15 weeks after transplantation of human FTHY/CD34+ HSPCs (n = 8) were stained with anti-human Hep par1 or CD45 antibodies. a Representative IHC staining images for microscopic areas enriched with (left panel) and lacked (right panel) human Hep par1+ cells (scale bar, 20 μm). Sections stained with anti-human Hep par1 and anti-human CD45 are shown in the top and bottom panels, respectively. b Pearson correlation between Hep par1+ and CD45+ cell numbers (measured in the original magnification × 200 photos). Correlation is significant at the 0.01 level (two-tailed), p < 0.0001, R = 0.678. c-g Liver tissues from LTH hu-mice (n = 8) and TH hu-mice (n = 2) were analyzed by multiplexed IHC assays using anti-human Hep par1 (cyan)/ CD68 (green)/CD11c (red)/CD94 (magenta) and DAPI (blue). c Representative staining images of liver tissues from LTH hu-mice (left and right panels show the microscopic areas enriched with and the areas lacking human Hep par1+ hepatocytes, respectively) and TH hu-mice were shown. Scale bars represent 50 μm (top) and 20 μm (bottom), respectively. d The densities (per mm2) of human CD68+, CD11c+ and CD94+ cells in the microscopic areas lacked (Hp−) or enriched with (Hp+) human Hep par1+ hepatocytes of liver tissues from LTH hu-mice, and the randomly selected areas in the liver tissues from TH hu-mice. Each symbol represents the density of positively stained cells in each individual microscopic area (10–12 areas were counted for each liver sample, and data are analyzed using unpaired, two-tailed Student’s t-test). e Densities of human CD68+, CD11c+ and CD94+ cells in the Hep par1− (Hp−) and Hep par1+ (Hp+) areas of liver tissues from LTH hu-mice. Data shown are the average densities of positively stained cells in the Hep par1- (Hp-) and Hep par1+ (Hp+) areas of each individual liver tissue (values from the same mouse liver tissue are connected with a line), and analyzed using paired, two-tailed Student’s t-test. ***p < 0.001; n.s. not significant