Fig. 6. FOXO1 regulated the expression of ARHGAP15.

a, b LoVo cells were transfected with FOXO1 interference or overexpression lentiviral vectors. The regulation of FOXO1 on the mRNA (a) and protein (b) expression of ARHGAP15 was assessed. c ARHGAP15 promoter sequence was cloned into pGL3-basic to construct the luciferase reporter plasmid. After transfected with the recombinant plasmid, luciferase reporter activity was detected. (d) Cell viability detected by CCK-8 assay to assess the antagonism between ARHGAP15 silencing and FOXO1 overexpression. Experiments were repeated three times independently. NS: no significant difference; *** P < 0.001