Skip to main content
. 2018 May 29;9:1182. doi: 10.3389/fimmu.2018.01182

Figure 1.

Figure 1

Aspergillus fumigatus and β-glucan induce neutrophil extracellular trap formation in human and murine neutrophils. (A) Representative images showing DNA release by SYTOX Green staining in human peripheral blood neutrophils 20 h after stimulation with 25 nM phorbol myristic acid (PMA), 100 μg/ml A. fumigatus hyphal extract (AspHE), or 100 μg/ml particulate β-glucan (curdlan); original magnification is 200×. (B) DNA release overtime shown as relative fluorescent units (RFU) measured by SYTOX Green; (C) total extracellular DNA after 20 h calculated as area under the curve (AUC). (D,E) Extracellular DNA from mouse peritoneal neutrophils incubated with AspHE or curdlan and quantified by SYTOX Green RFU and AUC. Asterisks (***) indicate p < 0.001 based on one way ANOVA with Tukey post hoc analysis. Experiments were repeated five times with similar results.