Figure 2.

Neutrophil extracellular trap (NET) formation is dependent on reactive oxygen species (ROS). Human neutrophils were stimulated 20 h with phorbol myristic acid (PMA), AspHE, or curdlan in the presence of the ROS inhibitor diphenyl iodonium (DPI) (10 μM). NET formation was quantified by SYTOX Green over time (A), and total SYTOX over 18 h is shown as area under the curve (AUC) (B). ANOVA with Tukey post hoc analysis showed ***p < 0.001 and **p < 0.01. Experiments were repeated four times with similar results.