Figure 3.

Distinct roles for CR3 and Dectin-1 in β-glucan-induced reactive oxygen species (ROS) production and neutrophil extracellular trap (NET) formation. (A–C) Human neutrophils; (D,E) murine neutrophils. Neutrophils were incubated with β-glucan (curdlan) in the presence of the anti-CR3 blocking antibody M1/70 (30 μg/ml) (A) or with the anti-Dectin-1 blocking antibody 22H8 (Invivogen, 20 μg/ml) (B). (A,B) DNA release was quantified by SYTOX Green and expressed as area under the curve. (C) ROS production measured by luminol. (D,E) DNA release and ROS production by mouse bone marrow neutrophils from C57BL/6, Dectin-1^−/−, and CD18^−/− mice stimulated with curdlan. p Values are biological replicates of at least three repeat experiments and were calculated using ANOVA with Tukey post hoc analysis (***p < 0.001, **p < 0.01, and *p < 0.05).