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. 2018 May 29;9:1182. doi: 10.3389/fimmu.2018.01182

Figure 5.

Figure 5

Protein deiminase 4 (PAD4)-dependent neutrophil extracellular trap formation. (A) Bone marrow neutrophils from C57BL/6 and PAD4−/− mice were incubated 20 h with particulate β-glucan (curdlan) in RPMI, and DNA release was quantified by SYTOX Green. Values were normalized to unstimulated neutrophils (RPMI). (B,C) Human peripheral blood neutrophils incubated with curdlan and 10 μM specific PAD4 inhibitor GSK484 (B) or with the related, non-inhibitory compound GSK106 (C). p Values are biological replicates of at least three repeat experiments and were calculated using ANOVA with Tukey post hoc analysis (***p < 0.01; **p < 0.05; ns, not significant). Experiments were repeated twice with similar results.