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CD18-dependent calprotectin (CP) (S100A8/A9) production. Bone marrow neutrophils from C57BL/6, PAD4−/−, and CD18−/− mice were incubated 2 or 16 h with curdlan, and CP/S100A8 was quantified by ELISA. p Values are biological replicates of three repeat experiments and were calculated using ANOVA using a Tukey post hoc analysis (***p < 0.001; **p < 0.01; ns, not significant). S100A8 production to AspHE was similar in all groups (Figure S1 in Supplementary Material).
