Figure 7.

CR3, but not protein deiminase 4 (PAD4), is required for neutrophil inhibition of Aspergillus fumigatus hyphal growth. (A,B) A. fumigatus hyphae incubated 18 h with human neutrophils in RPMI alone or with anti-CR3 M1/70 (20 μg/ml) (A). Fungal mass was quantified by calcofluor white fluorescent chitin stain and presented as percent of total hyphae in the absence of neutrophils. (B) Percent fungal mass in bone marrow neutrophils from C57BL/6, CD18^−/−, or PAD4^−/− mice following 18 h incubation with A. fumigatus hyphae. p Values are biological replicates of three repeat experiments and were calculated using Student’s t-test (A) or ANOVA with a Tukey post hoc analysis (B) (****p < 0.0001; ***p < 0.001; ns, not significant).