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. 2018 Apr 5;26(6):1494–1508. doi: 10.1016/j.ymthe.2018.04.002

Figure 3.

Figure 3

Ectopic Expression of CILA1 Enhances Cisplatin-Sensitive TSCC Cell Proliferation, Invasion, Anti-apoptosis, and EMT

(A) The overexpression efficiency was determined by real-time qPCR in cisplatin-sensitive TSCC cells. Quantitative real-time PCR (B), western blot (C), and immunofluorescence assays (D) were performed to examine the changes in EMT marker expression upon upregulation of CILA1. (E) Boyden chamber assays were explored to examine the changes in TSCC cell motility after CILA1 overexpression. Flow cytometry (F) and TUNEL assays (G) were performed to determine the anti-apoptotic effect of the CILA1 upregulation. (H) An MTS assay was performed to examine chemoresistant TSCC cell viability with overexpressed CILA1 (**p < 0.01; ***p < 0.001).