Figure 2.

Aux/IAA proteins and phytochrome A interact in vitro. Recombinant oat phyA (AsPhyA) and IAA proteins were mixed with streptavidin agarose beads, incubated at room temperature, and treated as described in “Materials and Methods.” Shown are SDS-PAGE resolved proteins of the binding reactions after Coomassie Blue staining for AsPhyA (A) and IAA protein (B and C) control incubations, as well as for the test incubations containing AsPhyA and IAA protein (D and E). S indicates the entire supernatant fraction after the first sedimentation step. P denotes the entire pellet fraction after the third washing step with detergent-containing buffer.