Figure 1.

Porcupine expression. (A) Porcn mRNA levels in different tissues from n mice. (B) Porcn mRNA levels in calvarial osteoblasts cultured with or without BMP2 for 7 days (n = 4 wells/condition and time point). Results are expressed as fold-change to the levels in control osteoblasts at day 2. As a positive control, we show Alpl mRNA levels at day 7. (C) Porcn mRNA levels in bone marrow cell-derived osteoclasts cultured with or without RANKL for 4 days (n = 4 wells/condition and time point). Results are expressed as fold-change to the levels in control osteoclasts at day 1. As a positive control, we show Acp5 mRNA levels at day 4. Bars represent the mean and error bars represent the 95% CI of the mean. P values are indicated as *P < 0.05; **P < 0.01; ***P < 0.001.