Figure 5.

Effects of Porcupine inhibition on trabecular bone. (A) Trabecular bone volume fraction (BV/TV) in L5 vertebral body assessed by µCT. (B) 3D reconstruction of 200 axial sections from an elliptic region of interest within trabecular bone of the L5 vertebra body from a representative animal treated with vehicle or LKG974 at 6 mg/kg/day (Bar: 200 µm). (C) BV/TV in the distal metaphyseal region of the femur assessed by µCT. (D) Bone histomorphometry at the trabecular bone of L5 vertebral body showing bone formation rate (BFR), mineralization apposition rate (MAR), mineralising surface over bone surface (MS/BS) and osteoclast number per bone surface (Oc.N). (E) Microscopy images of the trabecular bone from L5 vertebra body stained with TRAP showing multinucleated osteoclasts at the bone surface (Bar: 50 µm). (F) Alpl, Ctsk and Rankl/Opg ratio mRNA levels in trabecular bone. (G) Summary diagram of the effects of Porcupine inhibition on cortical or trabecular bone formation and resorption. For each graph, n = 10 animals per group. Bars represent the mean and error bars represent the 95% CI of the mean. P values are indicated as *P < 0.05; **P < 0.01; ***P < 0.001. Ps, periosteal; Ec, endocortical.