REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21 (DE3) – CAHS 77580	This study	NA
E. coli BL21 (DE3) – CAHS 89226	This study	NA
E. coli BL21 (DE3) – CAHS 94063	This study	NA
E. coli BL21 (DE3) – CAHS 106094	This study	NA
E. coli BL21 (DE3) – CAHS 107838	This study	NA
Chemicals, Peptides, and Recombinant Proteins
Trizol	ThermoFisher	15596026
Roche cOmplete EDTA-free protease inhibitor tablet	Sigma-Aldrich	4693159001
Lactate dehydrogenase	Sigma-Aldrich	10127230001
BSA	Sigma-Aldrich	B4287-25G
D-Trehalose	Sigma-Aldrich	6138-23-4
NADH	Sigma-Aldrich	10128023001
Critical Commercial Assays
RNeasy Mini Kit	QIAGEN	74104
TruSeq kit	Illumina	RS-122-2001
MasterPure RNA purification kit	Epicenter	MCR85102
Deposited Data
Assemblies generated in this study	NCBI BioProject database	PRJNA369152
Raw RNaseq reads generated in this study	NCBI Search Read Archive	SRR5217912, SRR5217911, SRR5217848, SRR5217652, SRR5217467
Experimental Models: Organisms/Strains
Hypsibius dujardini	Sciento	Z151
Paramacrobiotus richtersi	Collected for study	N/A
S. cerevisiae CAHS 94205	This study	N/A
S. cerevisiae CAHS 77580	This study	N/A
S. cerevisiae CAHS 68135	This study	N/A
S. cerevisiae CAHS 94063	This study	N/A
S. cerevisiae CAHS 106094	This study	N/A
Software and Algorithms
Trinity	Haas et al. (2013)	https://github.com/trinityrnaseq/trinityrnaseq/wiki
RSEM	Li and Dewey (2011)	http://deweylab.github.io/RSEM/
edgeR	Robinson et al. (2010)	https://bioconductor.org/packages/release/bioc/html/edgeR.html
CEGMA	Parra et al. (2007)	https://github.com/KorfLab/CEGMA_v2/
Excel 2008 for Mac V12.3	Microsoft	NA
Other
Tzero aluminum pans	Fisher	NC9553081