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Effects of ectopic ABI4 expression on ABA-inducible vegetative accumulation of transcripts normally expressed primarily in seeds. RNA was extracted from 13-d seedlings incubated for 2 d on plates ±50 μm ABA, then analyzed by RNA gel blots hybridized to cloned probes for the indicated transcripts. A, All lanes contain 10 μg of total RNA. B, All lanes contain 5 μg of RNA. C, Lanes with RNA from ABI3 ectopic expression line contain 1 μg of RNA; all others contain 10 μg of RNA.
