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. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Cell. 2018 May 10;173(5):1135–1149.e15. doi: 10.1016/j.cell.2018.04.013

Figure 1. VDR is essential for β cell homeostasis.

Figure 1

(A) Schematic representation of the genome-wide CRISPR loss-of-function screen in human iPSC-derived β-like cells.

(B) Gene ontology analysis of targets compromising β-like cell function (p<0.01, 156 total genes).

(C) p-value rank order plot of genes enriched in the loss-of-function screen, VDR is labeled in red.

(D) Distribution of normalized reads of individual sgRNAs in GFP sorted cells; VDR sgRNAs are labeled in red. X-axis location indicates sgRNA were found only in INS-GFP− cells.

(E) Immunohistochemistry staining of pro-insulin in VDR+/− or VDR−/− islets (scale bar: 100μm, each panel represents an individual mouse).

(F) Serum proinsulin/insulin ratio in VDR+/+ and VDR−/− male mice measured by ELISA (n=3, mean±S.E.M, * p<0.05).

See also Figure S1 and S2.