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. Author manuscript; available in PMC: 2019 May 17.
Published in final edited form as: Cell. 2018 May 10;173(5):1135–1149.e15. doi: 10.1016/j.cell.2018.04.013

Figure 3. BRD9 represses VDR activation upon acute cytokine stress in β cells.

Figure 3

(A) Heat map of VDR interactome in INS1 cells expressing HA-tagged VDR treated with vehicle, Cal, iBRD9, or Cal+iBRD9. Spectral counts normalized to VDR.

(B) Western blots of immunoprecipitates from INS1 cells expressing HA-tagged VDR with indicated treatments.

(C) Expression of Nfkbia in INS1 cells 30 minutes after exposure to IL1βand indicated co-treatments.

(D) Western bolt of NFKBIA (IκBα) in INS1 cells 30 minutes after exposure to IL1β with and without Cal, iBRD9, or Cal+iBRD9.

(E) Time course of Nfkbia expression in INS1 cells in response to IL1β with indicated treatments.

(F) Nfkbia levels in IL1β-stressed INS1 cells with siRNA knock down of PBRM1 or PCAF.

n=3, mean±S.E.M., *P<0.05

See also Figure S4.