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The expression of TTF-1 was inhibited after shRNA-TTF-1 transfection in rat ATII cells. Rat ATII cells were transfected with shRNA-TTF-1 or shRNA-scrambled. Twenty-four hours after transfection, the expression of TTF-1 was measured. (a) QPCR was used to analyze the mRNA expression of TTF-1. (b) Western blot was performed to analyze the protein expression of TTF-1. Quantitative data were presented as mean ± SEM (n = 5). ∗P < 0.05 compared with control.
