Table 1.
Synthesis and characterization of rHDL containing each ketohexose (fructose, tagatose, and psicose).
| rHDL | Molar composition | WMF (nm) | Sizea (Å) | Number of apoA-I/particleb | α-Helicity (%) |
|---|---|---|---|---|---|
| Native-A-I | 95 : 5 : 1 | 337 ± 0.1 (344 ± 0.5)c | 109–94 | 54.4 (32.6)c | |
| H2O-A-I | 95 : 5 : 1 | 335 ± 0.5 (344 ± 0.5) | 109–94 | 2, 3, 4 | 82.6 (51.4) |
| f-A-I | 95 : 5 : 1 | 342 ± 1.6 (344 ± 0.5) | 109–94 | 2, 3, 4, 5 | 38.4 (38) |
| t-A-I | 95 : 5 : 1 | 335 ± 1.2 (344 ± 0.5) | 93 | 2, 3, 4, 5 | 34.7 (15.4) |
| p-A-I | 95 : 5 : 1 | 335 ± 0.4 (344 ± 0.5) | 93 | 2, 3, 4, 5 | 35 (25.4) |
aDetermined from 8% to 25% native-gradient gel electrophoresis with densitometric scanning analysis. bDetermined from BS3-crosslinking and 8–25% SDS–PAGGE. cThe numbers in the parentheses indicate the proteins in the lipid-free state. H2O-A-I: H2O-treated apoA-I; f-A-I: fructose-treated apoA-I; t-A-I: tagatose-treated apoA-I; p-A-I: psicose-treated apoA-I.