Figure 1.

The figure illustrates the two experimental approaches used to humanize the zebrafish larvae. The composition of bacterial microbiota was analyzed through culture and MiSeq sequencing (A) Fecal sample was homogenized with pre-reduced PBS (0.1% cysteine) and allowed to stand for 5 min. The supernatant of the homogenized solution was used as inoculum to transplant 3 dpf zebrafish larvae. (B) Zebrafish larvae (3, 4, or 5 dpf) were inoculated by immersion with a suspension of each bacterium at a final concentration of 10⁸ CFU/mL in sterile E3 medium. La: Lactobacillus acidophilus, Ba: Bifidobacterium adolescentis, Cd: Clostridioides difficile, Bc: Bacillus clausii.