Figure 5.

Colonization of zebrafish larvae (3, 4, or 5 dpf) with human bacterial isolates. Bacterial strains were successively inoculated by immersion with a suspension of each bacterium at a final concentration of 10⁸ CFU/ml in sterile E3 medium (A) on day 3 dpf, (B) on day 3, 4, and 5 dpf. (C) Colonization of bacterial strains when they were inoculated alone. Bacterial colonization was determined by culture. (D) Viability of each bacterial strain in E3 medium. La: Lactobacillus acidophilus, Ba: Bifidobacterium adolescentis, Cd: Clostridioides difficile, Bc: Bacillus clausii. Each colonization experiment was performed independently at least 3 times; the results show mean ± SE and Mann-Whitney test performed.