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Effect of nutritional programming and offspring diet on mitochondrial dynamics in hypothalamus. Relative densitometry for protein levels of DRP1 (a, c) or MFN 2 (b, c) n = 8 per group. (c) Pictures are representative of Western Blot for DRP 1, MFN2 and Actin as loading control. mRNA expression levels of MFN2 (d), DRP 1 (e), Opa 1 (f) and Ip3r1 (g) n = 7 per group. Data are means ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001
