Figure 4.

Effect of C. campestris on morphological and functional properties of granulocyte in leukemic cells. NB4 cells were treated with C. campestris (12.5 µM) or As₂O₃ (0.5 µM) for 72 hr. Morphologic and functional properties of granulocytes were determined by Giemsa and NBT assays, respectively. In Giemsa-stained slides, As₂O₃-treated cells (as positive control) showed polymorphonuclear morphology of granulocyte and C. campestris-treated cells showed promyelocytes characteristics similar to control. On NBT slides, As₂O₃-treated cells (as positive control) showed intracellular blue-black formazan deposits while C. campestris-treated cells showed no intracellular blue-black formazan deposits (bar represents 0.01 mm