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. 2018 Jun 5;19:438. doi: 10.1186/s12864-018-4818-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Pyrosequencing analysis of genes with differentially methylated gene bodies. Four genes identified as having differentially methylated intragenic regions by the EDMA analysis were selected for pyrosequencing. All assays confirmed the directionality of the change in methylation at these loci between control samples and ART samples (a–e). DNA methylation was significantly lower in day 17 SOV TE samples, relative to in vivo controls (b).