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Fasting and postprandial C4 concentrations. C4, a valid marker of bile acid biosynthesis, was measured by high-performance liquid chromatography (HPLC). Comparison of C4 values between healthy controls (N = 16), overweight (N = 14) and obese (N = 12) patients with non-alcoholic fatty liver disease (NAFLD) at baseline (0 h), 2, 4 and 6 h after the oral fat tolerance test (OFTT). C4 concentrations did not differ between groups (Kruskal-Wallis-test),*outlier
