Table 3.
Overview of results from qPCR and ddPCR analyses for Gyrodactylus salaris (ITS), Oncorhynchus mykiss (CytB) and Salmo salar (CytB) at each sampling site. List of sampling sites including amount of water filtered, number of samples per site (each sample constitutes of one filter), the Cq value (from qPCR) and number of eDNA copies per litre (ddPCR) from all filters taken at each point, respectively. eDNA copies per litre are abbreviated as eDNA/l. No detection is indicated with a minus (-) for qPCR and a zero for ddPCR and those samples where analysis was not applicable are indicated with NT
| Site no. | Site name | Sample | Volume (l) | Gyrodactylus salaris | Oncorhynchus mykiss | Salmo salar | |||
|---|---|---|---|---|---|---|---|---|---|
| qPCRa | ddPCRb | qPCRa | ddPCRa | qPCR | ddPCRb, a | ||||
| 1 | Storåne at Ala camping | 1 | 1 | - | - | - | 0 | - | 0 |
| 2 | 1 | - | - | - | 0 | - | 0 | ||
| 2 | Storåne at Tørpegårdsvegen/bru | 1 | 1 | - | - | - | 0 | - | 0 |
| 2 | 1 | - | - | - | 0 | - | 0 | ||
| 3 | Trout farm | 1 | 1 | - | - | 17.48 | 7,848,000 | - | 0 |
| 2 | 1 | - | - | 17.50 | 8,800,000 | - | 0 | ||
| 4 | Leireelvi at Leira/Garlivegen | 1 | 1 | - | - | 29.62 | 1624 | - | 0 |
| 2 | 1 | - | - | 29.09 | 3816 | - | 0 | ||
| 5 | Leireelvi at Leira camping | 1 | 1 | - | - | 30.05 | 2240 | - | 0 |
| 2 | 1 | - | - | 30.02 | 2124 | - | 0 | ||
| 6 | Lake Strondafjorden at Faslefoss | 1 | 1 | - | - | 32.3 | 560 | - | 0 |
| 2 | 1 | - | - | 31.68 | 576 | - | 0 | ||
| 7 | River Begna at Bagn | 1 | 1 | - | - | > cut-offc | 0 | - | 0 |
| 2 | 1 | - | - | 36.91 | 22 | - | 0 | ||
| 8 | River Begna at Nes | 1 | 1 | - | - | - | 0 | - | 0 |
| 2 | 1 | - | - | - | 0 | - | 0 | ||
| 9 | River Lierelva at Sjåstad | 1 | 1 | 34.52 | 560 | - | NT | NT | 9200 |
| 2 | 1 | 33.56 | 840 | - | NT | NT | 10,160 | ||
| 3 | 1 | 33.94 | 864 | - | NT | NT | 7520 | ||
| 4 | 1 | 24.89 | 371,440 | - | NT | NT | 8912 | ||
aRun as singleplex
bRun as duplex
cCut-off value was set at Cq 41