Fig. 3.

a. The sequences in UTR region of AK4 gene targeted by miR-199a-3p. b-e. The relative luciferase activity (fold) of the reporter with wild-type (WT) AK4-UTR or with no UTR (Vec) were determined in the miR-199a-3p mimic (in G-292 and U2OS) or antagomiR (in MNNG/HOS) or Mock transfected osteosarcoma cells. The renilla luciferase activity of a co-transfected control plasmid was used to control the transfection efficacy. The representative results from three independent experiments are shown. *P<0.05; **P<0.01 by Student’s t-test