Fig. 4.

a. The levels by qRT-PCR of AK4 mRNAs in the mimic (3 PM) and siRNAs transfected G-292 and U2OS cells versus the NC. b. The AK4 protein level (western blot analysis) in the 3 PM and siRNAs transfected versus the NC transfected G-292 cells. c. The relative cell survival of the G-292 cells transfected by 3 PM and siRNAs over the NC transfected G-292 cells, 72 h after a treatment of the IC₅₀ dosed drugs. d. The relative cell survival of the U2OS cells transfected by 3 PM and siRNAs over the NC transfected U2OS cells, 72 h after a treatment of the IC₅₀ dosed drugs. e. The relative cell survival of the MNNG/HOS cells transfected by miR-199a-3p antagomiR (3PA) over the NC transfected MNNG/HOS cells, assayed at 72 h post-treatment of the IC₅₀ dosed drugs. *P value < 0.05; **P value < 0.01