Figure. 5.

Small interfering RNA (siRNA) attenuate propionic acid-mediated t-PA induction in NHBE cells. (A, B) Submerged NHBE cells were transfected with 40 nmol/L of control siRNA and siRNA targeting human GPR41 (A) or GPR43 (B). Whole cell lysates were electrophoresed and transferred onto a PVDF membrane. Representative Western blot with anti-human GPR41 or anti-human GPR43 antibody is shown (bottom). Semiquantitative densitometry of 4 independent experiments. (C, D, E) Submerged NHBE cells were transfected with 40 nmol/L of control siRNA or siRNA targeting human GPR41 (C), GPR43 (D), or GPR41 and GPR43 (E) as indicated. Forty-eight hours after transfection, cells were stimulated with 10mM of propionic acid for 24 h (n = 6). Data shown are mean ± SEM of independent experiments. *P < .05, ** P < .01, ***P < .001